PUBLICATIONS
Key Publications
Preprints
2023
2022
Le P, Kim J, Koehler KR†*. The mechanical forces shaping our senses. Development. 2022 April, dev197947.
Lee J, van der Valk WH, Serdy SA, Deakin C, Kim J, Le AP, Koehler KR†*. Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells. Nature Protocols. 2022 May, 1266–1305.
Tang PC*, Chen L, Singh S, Groves AK, Koehler KR, Liu XZ, Nelson R†*. Wnt signaling promotes cell caudalization and inner ear differentiation in mouse stem cell-derived organoids. Stem Cells, 2022.
Nist-Lund C, Kim J, Koehler KR†*. Advancements in inner ear development, regeneration, and repair through otic organoids. Current Opinion in Genetics & Development. 2022 July, 101954.
Lee J, Koehler KR†*. Skin organoids: A new human model for developmental and translational research. Exp Dermatol. 2021 Apr;30(4):613-620. doi: 10.1111/exd.14292. Epub 2021 Feb 18. PubMed PMID: 33507537; PubMed Central PMCID: PMC8265774.
Nourbakhsh A, Colbert BM, Nisenbaum E, El-Amraoui A, Dykxhoorn DM, Koehler KR, Chen ZY, Liu XZ. Stem Cells and Gene Therapy in Progressive Hearing Loss: the State of the Art. J Assoc Res Otolaryngol. 2021 Apr;22(2):95-105. doi: 10.1007/s10162-020-00781-0. Epub 2021 Jan 28. Review. PubMed PMID: 33507440; PubMed Central PMCID: PMC7943682.
2021
2020
Koehler Lab team members are highlighted in bold
For a full list of publications, visit PubMed here
van der Valk WH, Steinhart MR, Zhang J, Koehler KR†*. Building inner ears: recent advances and future challenges for in vitro organoid systems. Cell Death Differ. 2021 Jan;28(1):24-34. doi: 10.1038/s41418-020-00678-8. Epub 2020 Dec 14. Review. PubMed PMID: 33318601; PubMed Central PMCID: PMC7853146.
Arkenberg MR, Dimmitt NH, Johnson HC, Koehler KR, Lin CC. Dynamic Click Hydrogels for Xeno-Free Culture of Induced Pluripotent Stem Cells. Adv Biosyst. 2020 Nov;4(11):e2000129. doi: 10.1002/adbi.202000129. Epub 2020 Sep 13. PubMed PMID: 32924337; PubMed Central PMCID: PMC7704730.
Lee J, Rabbani C, Gao H, Steinhart M, Woodruff BW, Pflum Z, Kim A, Heller S, Liu Y, Shipchandler TZ, Koehler KR†*. Hair-bearing human skin generated entirely from pluripotent stem cells. Nature, 2020, June; doi: https://doi.org/10.1038/s41586-020-2352-3
Tang PC, Alex AL, Nie J, Lee J, Roth AA, Booth KT, Koehler KR, Hashino E, Nelson R. Defective Tmprss3-associated hair cell degeneration in inner ear organoids. Stem Cell Reports 13, 147-162.
Lee J, Boescke R, Tang PC, Hartman BH, Heller S, Koehler KR†*. Hair follicle development in mouse pluripotent stem cell-derived skin organoids. Cell Reports 22, 242-254 (†Lead Contact, *Corresponding Author, Highlighted in Nature and on NPR's Science Friday)
Koehler KR†*, Nie J, Longworth-Mills E, Liu XP, Lee J, Holt JR, Hashino E*. Generation of inner ear organoids with functional hair cells from human pluripotent stem cells. Nature Biotechnology 480, 547. http://doi.org/10.1038/nbt.3840 (†Lead Contact, *Corresponding Author, Cover Image, Highlighted in NBT News and Views and Cell Stem Cell Previews)
Nie J, Koehler KR, Hashino E. Directed Differentiation of Mouse Embryonic Stem Cells Into Inner Ear Sensory Epithelia in 3D Culture. Methods in Molecular Biology 1597, 67-83.
DeJonge RE, Liu XP, Deig CR, Heller S, Koehler KR*, Hashino E*. Modulation of Wnt signaling enhances inner ear organoid development in 3D culture. PloS One, 11(9), e0162508. http://doi.org/10.1371/journal.pone.0162508 (*Corresponding Author)
Liu XP, Koehler KR, Mikosz AM, Hashino E, Holt JR. Functional development of mechanosensitive hair cells in stem cell-derived organoids parallels native vestibular hair cells. Nature Communications, 7, 11508. http://doi.org/10.1038/ncomms11508
Shimomura A, Patel D, Wilson SM, Koehler KR, Khanna R, Hashino E. Tlx3 promotes glutamatergic neuronal subtype specification through direct interactions with the chromatin modifier CBP. PloS One 10(8):e0135060.
Koehler KR*, Hashino E*. 3D mouse embryonic stem cell culture for generating inner ear organoids. Nature Protocols 9(6):1229–1244 (*Corresponding Author; Cover Image)
Koehler KR*, Mikosz AM, Molosh AI, Patel D, Hashino E*. Generation of inner ear sensory epithelia in a defined 3D culture. Nature 500(7461):217-221 (*Corresponding Author; Highlighted in Cell, BioTechniques, 8-Star F1000 recommendation, and Nature Neuroscience’s NeuroPod)
Koehler KR, Tropel P, Theile JW, Kondo T, Cummins TR, Viville S, Hashino E. Extended passaging increases the efficiency of neural differentiation of induced pluripotent stem cells. BMC Neuroscience 12(1):82-95
2019
2018
2017
2016
2015
2014
2013
2011
Gopee NH, Huang N, Olabi B, Admane C, Botting RA, Foster AR, Torabi F, Winheim E, Sumanaweera D, Goh I, Miah M, Stephenson E, Tun WM, Moghimi P, Rumney B, He P, Lawrence S, Roberts K, Sidhpura K, Englebert J, Jardine L, Reynolds G, Rose A, Ganier C, Rowe V, Pritchard S, Mulas I, Fletcher J, Popescu DM, Poyner E, Dubois A, Filby A, Lisgo S, Barker RA, Park JE, Vento-Tormo R, Le PA, Serdy S, Kim J, Deakin C, Lee J, Nikolova M, Rajan N, Ballereau S, Li T, Moore J, Horsfall D, Lozada DB, O’Toole EA, Treutlein B, Bayraktar O, Kasper M, Mazin P, Gambardella L, Koehler KR*, Teichmann SA*, Haniffa M*. A human prenatal skin cell atlas reveals immune cell regulation of skin morphogenesis. bioRxiv. 2023;2023.10.12.556307.
Zhang J, van der Valk WH, Steinhart MR, Nist-Lund C, Lee J, Koehler KR. A simplified method for generating inner ear organoids from pluripotent stem cells. Protocol Exchange.
Sounak Sahu, Sarthak Sahoo, Teresa Sulliva, T. Norene O’Sullivan, Sevilay Turan, Mary E. Albaugh, Sandra Burkett, Bao Tran, David S. Salomon, Serguei V. Kozlov, Koehler KR†*, Mohit Kumar Jolly, Shyam K. Sharan. Spatiotemporal modulation of growth factors directs the generation of multilineage mouse embryonic stem cell-derived mammary organoids. Developmental Cell, (2023)
Sandoval AGW, Gim KY, Huang J, Koehler KR†*. Applications of Human Pluripotent Stem Cell-Derived Skin Organoids in Dermatology. J. Investig. Dermatol. 143, 1872–1876 (2023).
Steinhart MR, van der Valk WH, Osorio D, Serdy SA, Zhang J, Nist-Lund C, Sun L, Lee J†*, Koehler KR†*. Mapping oto-pharyngeal development in a human inner ear organoid model. Development 150, (2023).
Doda D, Jimenez SA, Rehrauer H, Carreño JF, Valsamides V, Di Santo S, Widmer HR, Edge A, Locher H, van der Valk WH, Zhang J, Koehler KR, Roccio M†*. Human pluripotent stem cells-derived inner ear organoids recapitulate otic development in vitro. Development 150, (2023).
van der Valk WH, Beelen ESA van, Steinhart MR, Nist-Lund C, Osorio D, Groot JCMJ de, Sun L, Benthem PPG van, Koehler KR†*, Locher H†*. A single-cell level comparison of human inner ear organoids with the human cochlea and vestibular organs. Cell Rep. 2023;42(6):112623. PMID: 37289589